2011.06.25 01:34 "Re: [Tiff] 16-bit image", by Leandro Bugnon

 thought that saving in TIFF file format would ensure that all values I
save, they'll show in the exact same way.

The only solution that I can think about is make a calibration image as the first one, defining the min and max values. This isn't very atractive, but I have to use ImageJ becouse there we can develope all the processing task.

Maybe I should try some variation, like OME-TIFF, becouse some parameters cant be saved with tags, like the Z Resolution.

Thank you again,

2011/6/24 Bob Friesenhahn <bfriesen@simple.dallas.tx.us>

On Fri, 24 Jun 2011, Leandro Bugnon wrote:

> The image was made that way intentionally. My problem is that if I have a

stack with 2 images or slices, some of the viewers I've tryed define the display range between the min and max of the first image. In the test image,

>> the value 500 is represented in black and 700 in white, so the second >> image (1000-1400) it's completely white.

I cannot scale the image becouse it's for cuantitative microscopy, and the values goes from 0 to 2^14, so if I have a image from 500-700, it have to be almost dark and darker than the second image. I supposed that had some tag missed, but it seems not. The pixel values are correctly saved, the problems seems the visualization info to show them in a scale from 0 to 2^16 ( I can scale from 0-2^14 to 0-2^16 but I cannot scale anything to 0-2^16 becouse I'm distorting the information).

The traditional sort of image viewers you tried (imageJ and irfanview) are really intended for images scaled to ranges for normal viewing on

an 8-bit display. You will need a scientific/remote-sensing sort of display application to make sense of your images.

> bfriesen@simple.dallas.tx.us, http://www.simplesystems.org/** > users/bfriesen/ <http://www.simplesystems.org/users/bfriesen/>

GraphicsMagick Maintainer, http://www.GraphicsMagick.org/

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Leandro