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March 2006
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2006.04.02 17:15 "Re: 16-bit TIFF to 8-bit BMP/TIF ???", by David Scriven
Chris, There are quite a few packages, depending on your platform, that display 16bit images without conversion. NIH Image (Mac) ImageJ (all platforms) ImageMagic (Linux, maybe others) ScionImage (Windows). I'm sure there are others. Some packages require that you 'import' 16 bit images rather 'open' them to view them. I'm not sure why you have the need to convert the images to 8-bit to analyse them, as the whole point of 16 bit is increased dynamic range and finer granularity. Conversion to 8-bit removes that, and may distort the intensity relationships. For example, taking the square root will not result in the same values as dividing by 256 (say). libtiff handles 16 bit images as easily as 8 bit and once you've extracted the data, the analysis protocols should be the same.