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March 2006
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2006.03.31 05:49 "16-bit TIFF to 8-bit BMP/TIF ???", by Chris Le Brese
Hi, I am in the process of writing a program using openCV that analyses 8 bit images. The problem I have is that images produced from a fluorescent camera are 16 bit unsigned greyscale TIFF images (industry standard). This is a major project for University (DNA Microarray Image Analysis) I would like to convert this image to an 8 bit greyscale image so that I can analyse and display the image with intensities in proportion with the original image. The way I would like to do this is to get every pixels intensity value (16 bit) and Square root it to bring it into the range between 0 and 255. I am very much a newbie to the LibTIF package so any help will be greatly appreciated. I would like to steer away from using other programs to accomplish this task so I can combine it into the analysis program. This part is pretty urgent. The analysis is complete I am now trying to get TIFF images working with it. Regards, Chris Le Brese